diff --git a/pkgs/development/python-modules/intreehooks/default.nix b/pkgs/development/python-modules/intreehooks/default.nix
deleted file mode 100644
index cec0f2a7120d..000000000000
--- a/pkgs/development/python-modules/intreehooks/default.nix
+++ /dev/null
@@ -1,28 +0,0 @@
-{ lib
-, buildPythonPackage
-, fetchPypi
-, pytoml
-, pytest
-}:
-
-buildPythonPackage rec {
-  pname = "intreehooks";
-  version = "1.0";
-  format = "setuptools";
-
-  src = fetchPypi {
-    inherit pname version;
-    sha256 = "87e600d3b16b97ed219c078681260639e77ef5a17c0e0dbdd5a302f99b4e34e1";
-  };
-
-  propagatedBuildInputs = [ pytoml ];
-
-  nativeCheckInputs = [ pytest ];
-
-  meta = {
-    description = "Load a PEP 517 backend from inside the source tree";
-    license = lib.licenses.mit;
-    maintainers = [ lib.maintainers.fridh ];
-    homepage = "https://github.com/takluyver/intreehooks";
-  };
-}
diff --git a/pkgs/top-level/python-aliases.nix b/pkgs/top-level/python-aliases.nix
index 719fb88362ab..35079718420c 100644
--- a/pkgs/top-level/python-aliases.nix
+++ b/pkgs/top-level/python-aliases.nix
@@ -124,6 +124,7 @@ mapAliases ({
   hyperkitty = throw "Please use pkgs.mailmanPackages.hyperkitty"; # added 2022-04-29
   IMAPClient = imapclient; # added 2021-10-28
   imdbpy = throw "imdbpy has been renamed to cinemagoer"; # added 2022-08-08
+  intreehook =  throw "intreehooks has been removed because it is obsolete as a backend-path key was added to PEP 517"; # added 2023-04-11
   ipaddress = throw "ipaddress has been removed because it is no longer required since python 2.7."; # added 2022-05-30
   influxgraph = throw "influxgraph has been removed because it is no longer maintained"; # added 2022-07-10
   itanium_demangler = itanium-demangler; # added 2022-1017
diff --git a/pkgs/top-level/python-packages.nix b/pkgs/top-level/python-packages.nix
index c62e970ac92a..f2bb3202f356 100644
--- a/pkgs/top-level/python-packages.nix
+++ b/pkgs/top-level/python-packages.nix
@@ -4802,8 +4802,6 @@ self: super: with self; {
 
   into-dbus-python = callPackage ../development/python-modules/into-dbus-python { };
 
-  intreehooks = callPackage ../development/python-modules/intreehooks { };
-
   invisible-watermark = callPackage ../development/python-modules/invisible-watermark { };
 
   invocations = callPackage ../development/python-modules/invocations { };