diff --git a/pkgs/applications/science/biology/samtools/default.nix b/pkgs/applications/science/biology/samtools/default.nix
index 20d9565bce44..99210092a154 100644
--- a/pkgs/applications/science/biology/samtools/default.nix
+++ b/pkgs/applications/science/biology/samtools/default.nix
@@ -2,11 +2,11 @@
 
 stdenv.mkDerivation rec {
   pname = "samtools";
-  version = "1.11";
+  version = "1.13";
 
   src = fetchurl {
     url = "https://github.com/samtools/samtools/releases/download/${version}/${pname}-${version}.tar.bz2";
-    sha256 = "1dp5wknak4arnw5ghhif9mmljlfnw5bgm91wib7z0j8wdjywx0z2";
+    sha256 = "sha256-YWyi4FHMgAmh6cAc/Yx8r4twkW3f9m87dpFAeUZfjGA=";
   };
 
   nativeBuildInputs = [ perl ];
diff --git a/pkgs/development/libraries/science/biology/htslib/default.nix b/pkgs/development/libraries/science/biology/htslib/default.nix
index 3233a29449aa..52822baca631 100644
--- a/pkgs/development/libraries/science/biology/htslib/default.nix
+++ b/pkgs/development/libraries/science/biology/htslib/default.nix
@@ -2,11 +2,11 @@
 
 stdenv.mkDerivation rec {
   pname = "htslib";
-  version = "1.11";
+  version = "1.13";
 
   src = fetchurl {
     url = "https://github.com/samtools/htslib/releases/download/${version}/${pname}-${version}.tar.bz2";
-    sha256 = "1mrq4mihzx37yqhj3sfz6da6mw49niia808bzsw2gkkgmadxvyng";
+    sha256 = "sha256-8kB9+fl/C7awdlZXnkGhylEARkBntrIb+WKi6ksO/WU=";
   };
 
   # perl is only used during the check phase.
diff --git a/pkgs/development/python-modules/pysam/default.nix b/pkgs/development/python-modules/pysam/default.nix
index 31e01e83dbf6..41cdcad20385 100644
--- a/pkgs/development/python-modules/pysam/default.nix
+++ b/pkgs/development/python-modules/pysam/default.nix
@@ -15,7 +15,7 @@
 
 buildPythonPackage rec {
   pname   = "pysam";
-  version = "0.16.0.1";
+  version = "0.17.0";
 
   # Fetching from GitHub instead of PyPi cause the 0.13 src release on PyPi is
   # missing some files which cause test failures.
@@ -24,7 +24,7 @@ buildPythonPackage rec {
     owner = "pysam-developers";
     repo = "pysam";
     rev = "v${version}";
-    sha256 = "168bwwm8c2k22m7paip8q0yajyl7xdxgnik0bgjl7rhqg0majz0f";
+    sha256 = "sha256-RDeBq6pwBGCBNIn8YOPQr96GuL6FKEYeLAPQD6XN0iE=";
   };
 
   nativeBuildInputs = [ samtools ];